A newly published study led by researchers from the University of Georgia and several partner institutions reveals a discovery that could lead to new control strategies for a tiny-but-persistent agricultural pest that causes enormous soybean losses.Microscopic soybean cyst nematodes (SCN) Iive in soil and are attracted to the root systems of soybeans. Once a field is infested, it is nearly impossible to root them out, said lead study investigator Melissa Mitchum, professor of plant nematology in UGA’s Department of Plant Pathology and Institute of Plant Breeding, Genetics and Genomics (IPBGG) in the College of Agricultural and Environmental Sciences and Center for Applied Genetic Technologies.Invisible to the naked eye, SCN have unique hollow, protrudable mouth spears — called stylets — that they use to pierce through the root of a plant, injecting peptide effectors, which mimic the soybean’s naturally present CLAVATA3/embryo surrounding region (CLE) peptides to hijack the plant.After hatching from an egg in the soil, the juvenile nematode migrates into the root, where it sets up a feeding site and makes its way toward the circulatory system that the plant uses to transport nutrients to the rest of the plant.Once a root cell is chosen for feeding, the nematodes transform it into a syncytium, a mass comprised of hundreds of metabolically active cells that continue to secrete CLE, taking over the plant’s natural cellular processes to export the peptides back out of the cell to function as external signaling molecules to distant cells.This process diverts resources away from the plant in support of the nutrient demands of the developing nematode. At this point, nematodes change their morphology, turning from wormlike forms into sedentary, lemon-shaped bodies that pop out of the roots, becoming visible to the eye.Study resultsUsing knowledge that has been developed over the past two decades on how nematode CLEs function, the study, published in the journal New Phytologist, uncovered a new pathway that allows the nematode to disrupt the plant’s natural growth processes to divert resources for its own benefit. The study was also highlighted in commentary by the editors of New Phytologist. “We’ve discovered that early on, when the nematode delivers the CLE peptide to a root cell, the peptide has to reach a plant receptor that binds it so that you get a response in the plant. We knew that the nematode delivered the peptide into the cytoplasm of the cell, but the receptor is on the outside of the cell,” Mitchum said.The study shows that the nematode CLEs provide a way for the nematode peptide to get outside of the cell to interact with the plant protein receptor through the plant’s secretion system, a process that is not well-studied in plants.“To me it is fascinating because the nematodes have discovered how to co-opt that part of the plant — its own secretion system — but we don’t understand the secretion system they are taking advantage of,” Mitchum said. “If we can understand how they are doing it, we will likely uncover some novel aspect of plant biology that could have importance to other plant pathosystems. This effector protein is helping us understand plant biology, not just plant pathology.”Normally occurring plant CLE peptides regulate aspects of cell differentiation in the plant, communicating to the plant’s stem cells, which are constantly regenerating, whether to develop into a root cell, a leaf cell or a cell in another part of the plant.“The peptides in the plant — and there are many types — tell the plant how to regulate its growth and development. These nematodes have found a way to trick the plant by secreting these similar peptides in the root cell to make a feeding site,” Mitchum said. “Now we want to determine how the nematode CLE peptide forms that feeding site. That is why it is such a successful parasite — it has evolved this peptide that looks like and functions like a plant peptide, so they can put it into the root cell where it does not normally occur and trick the plant into supporting the formation of a feeding cell for the nematode.”Developing new control strategiesThe next step is to find novel methods to block the nematode peptides from getting out of the feeding cells.“We are trying to figure out how these nematode peptides function because we want to devise a way to interfere with that ability. If we can block the nematode peptides from getting out of the feeding cells, they will not form properly, and the nematode loses its nutrient source and dies,” Mitchum said. “If we can stop the nematodes from making those peptides or keep them from interfering with the processes in the cell, we can engineer different methods of resistance. That is why we study at this detailed level.”Soybean cyst nematodes are the No. 1 yield-reducing pest of soybean producers, robbing seemingly healthy fields of up to 30% of their yields — and costing the industry up to $1 billion every year.The primary management practice for soybean cyst nematodes is through developing genetic resistance in soybean varieties. Because SCN has a specialized host range, producers can control nematode damage by rotating fields to a non-host crop plant to reduce the population of nematodes in the field. However, the bodies of dead female nematodes form a cyst around them, protecting hundreds of eggs. These cysts can remain in the ground for years, protecting the eggs until a host crop is planted again and the nematodes reemerge.A troubling trendWhile creating genetically resistant crops is a very effective tool, SCN have adapted to overcome the source of resistance in the commonly used breeding line PI 88788, which is used in more than 90% of resistant varieties currently planted by producers. As a result, yields in resistant varieties can be reduced by up to 14 bushels per acre, according to The SCN Coalition, a public/private partnership of university researchers, national extension specialists and agriculture company representatives who are concerned about the evolving threat from soybean cyst nematodes.“We need to bring awareness to the increased resistance of SCN to the (PI 88788) resistance and come up with novel approaches to combat it. Targeting the mechanism we have found in this study would be a novel way of addressing this resistance,” she said.Because the symptoms of SCN infestation are often not visible above ground, it is difficult to communicate the severity of the problem to producers and the importance of basic science research to combat it.“If farmers are repeatedly planting the same resistant varieties, which most of them are, and they don’t realize what is going on below ground, the nematodes eventually become resistant to the resistant varieties. Over the past 20 to 30 years, soybean cyst nematodes have shifted from being unable to reproduce in these varieties to nematode populations that are highly aggressive and population densities are going up,” Mitchum said. “The only way growers can know they have a problem is to send in a soil sample to a diagnostic lab. This usually only occurs if they see aboveground symptoms and want to know if they have an issue, but these nematodes can be causing yield loss in the absence of any aboveground symptoms.”Mitchum says there are multiple potential targets to engineer new SCN resistance through the pathway they’ve discovered. Consequently, Mitchum and colleagues have filed several patents related to the research described in this study.“In order to disrupt this target, we can modify the receptor in the plant to retain its function in plant growth and development, but so it does not recognize nematode CLEs; we can try to block the ability of the nematode peptide to be trafficked through the plant’s secretion system; or we can prevent the nematode from making and secreting CLEs,” she said. “There are multiple ways to engineer resistance through this one pathway. If we can knock out a receptor, we can reduce infection, but because the nematode is co-opting a normal growth and development pathway, we have to do it in a way that is not disrupting plant development. This is a highly evolved interaction and we have to be able to understand it to precisely tweak it. We have to do it in a very targeted and specific way.”Assessing the threatBecause soybean cyst nematodes are such a widespread pest, Mitchum and UGA Extension plant pathologist Bob Kemerait, with the help of county agents, will launch a statewide survey in 2021 of all soybean producing counties in Georgia to determine current SCN levels and whether SCN-resistant crops are suffering greater losses due to the nematode’s adaptations against resistant varieties.While Georgia is not a major producer of soybeans — with 100,000 acres planted in 2019 at a value of about $240,000— more than 80 million acres were planted nationwide in 2019, with a production value of $31.2 billion, according to the U.S. Department of Agriculture’s National Agriculture Statistics Service.“From a genetic standpoint, we are working with soybean breeders to diversify the germplasm and working with companies to get new SCN-resistant varieties out to producers while, in parallel, we are doing this kind of basic science research to understand the mechanisms used by the nematode to cause disease so we can interfere with them in a novel way,” Mitchum said.Zeroing in on the scienceBringing awareness to the importance of basic science and basic research is a message that needs to come through when discoveries like this are made, Mitchum said.“A lot of times the return on research investment is not as immediate or apparent as it is if you go out and do a field trial and get immediate results,” Mitchum said. “There has to be a balance — we need to invest in research that will have an immediate impact, but if we are going to come up with breakthrough technologies, it is going to be from an investment in this kind of long-term research.”Lead study author Jianying Wang was a former research associate in Mitchum’s lab. Research partners in the multi-institutional study included Richard S. Hussey, Emeritus Distinguished Research Professor of Nematology at CAES; and CAGT postdoctoral researcher Xunliang Liu; as well as colleagues from University of Missouri, Worcester Polytechnic Institute, Iowa State University, North Carolina State University and Cornell University. Mitchum joined UGA in 2019 from University of Missouri’s Division of Plant Sciences and Bond Life Sciences Center.Funding for this work was from the National Science Foundation and the USDA National Institute of Food and Agriculture’s Agriculture and Food Research Initiative.For more information on research being performed at CAES, visit caes.uga.edu/research.
Joint venture to build 500MW of wind and solar capacity in Bangladesh by 2023 FacebookTwitterLinkedInEmailPrint分享The Business Standard:Bangladesh-China Power Company Limited (BCPCL), a joint venture of North West Power Generation Company Ltd (NWPGCL) and China National Machinery Import and Export Corporation (CMC), is going to implement renewable energy projects with a target to generate 500 megawatts (MW) of electricity across the country by 2023.As part of the initiative, they signed a joint-venture agreement to form a company named Bangladesh-China Power Company Ltd (Renewable) on Tuesday.In the newly formed joint venture, NWPGCL and CMC will be investing equal amounts of capital. The authorised capital of the joint-venture company is Tk1,000 crore while the paid-up capital is Tk16 crore.BCPCL (Renewable) will implement three solar plants – Pabna 60MW, Sirajganj 100MW and Jamuna 125MW – and one wind plant. The wind plant with a capacity of 50MW has been planned to be set up in Payra of Patuakhali. Apart from these, solar and wind plants having 165MW capacity will be implemented in different areas of the country which are yet to be finalised.State Minister for Power, Energy and Mineral Resources Nasrul Hamid said the planned projects will help the government meet the target to generate 10 percent of energy from renewable sources.More: Bangladesh-China Power Company to implement 500MW renewable energy projects
Think Then · Junior Gabriella Then and the No. 1 Women of Troy won the inaugural East Lake Cup in Atlanta, Georgia on Tuesday. Then won her semifinal match against Baylor and followed with a win in the finals against Duke. – Photo courtesy of Sports InformationThe No. 1 women’s golf team closed out a 4-1 semifinal victory over Baylor at 4:30 a.m. on Tuesday and followed it with a 4-1 win over Duke to clinch the first-ever East Lake Cup in Atlanta, Georgia.The Women of Troy captured their second first-place finish of the year in four fall events.Senior Kyung Kim won her match Monday night while freshman Robynn Ree, junior Gabriella Then and junior Tiffany Chan clinched wins Tuesday. Junior Karen Chung was the lone Woman of Troy to fall to Baylor.Chung redeemed her semifinal loss with a win over her Duke opponent in the final match. Kim, Then and Chan won as well, while Ree dropped her match to Duke.The Women of Troy will not play again until Feb. 7 at the Northrop Grumman Regional Challenge.The men’s team defeated LSU 4-1 in the third-place match of the East Lake Cup after falling 4-1 to Illinois in the semifinals.Freshman Justin Suh, sophomore Sean Crocker, junior Rico Hoey and sophomore Jonah Texeira all tallied wins over LSU to propel the Trojans to a third-place finish in Atlanta.“Even though we were in the consolation match, it was nice to redeem ourselves with LSU,” Hoey said. “We didn’t show them what we were capable of at nationals, so it was good to play to our potential today and get a win.”The Trojans will play again on Feb. 4 at the Amer Ari Invitational in Waikoloa, Hawaii.
Ireland will head into next week’s clash with Georgia with a spring in their step following last night’s 29-15 win over the Springboks at the Aviva.Joe Schmidt’s side showed great defensive work, tactical awareness and spirit, as they proved too much for the second-ranked side in the world to handle.Rhys Ruddock and Tommy Bowe got the Ireland tries, with man-of-the-match Jonathan Sexton kicking two conversions and four penalties.